Home > Sandwich ELISA Kits > Human Sialic Acid Binding Ig Like Lectin 6 (SIGLEC6) ELISA Kit

Product Name:

Human Sialic Acid Binding Ig Like Lectin 6 (SIGLEC6) ELISA Kit

Cat.#:

JL32268

Regulatory Status:

RUO

Alternative:

CD33L; CD33L1; OBBP1; OB-BP1; SIGLEC-6; CD327; CD33 antigen-like 1; Obesity-binding protein 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.075ng/mL

Specificity:

This kit recognizes Human SIGLEC6 in samples. No significant cross-reactivity or interference between Human SIGLEC6 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SIGLEC6

Sialic acid-binding Ig-like lectin 6 (SIGLEC6) is a protein-coding gene. Diseases associated with SIGLEC6 include non-gestational choriocarcinoma. Its related pathways include immunoregulatory interactions between lymphocytes and non-lymphocytes and the innate immune system.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SIGLEC6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-102	96
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human SIGLEC6 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	90-110
1 : 2	Average (%)	91	96
1 : 4	Range (%)	89-103	105-115
1 : 4	Average (%)	94	107